(2) The units of volume and concentration must be the same respectively. (1) Three of the 4 values must be known. Basic Rules for Solving C1V1 C2V2 Problems.
Add 40 mL of autoclaved ddH2O and begin mixing. Weigh out the mass of EDTA into a beaker 4. Find mass of EDTA using moles and MW 3. Use the Molarity formula to find moles 2.
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Invert bottle 2-3X to mix, then aliquot into 15 mL Falcon tubes and store in the freezer. 3.1M) Calculate amount of 1M Tris-HCL to add using C1V1=C2V2 Add appropriate amount of autoclaved ddH2O.4.
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